Journal: PLoS ONE

Article Title: RNA of Enterococcus faecalis Strain EC-12 Is a Major Component Inducing Interleukin-12 Production from Human Monocytic Cells

doi: 10.1371/journal.pone.0129806

Figure Lengend Snippet: Heat-killed L . gasseri JCM5344, E . faecalis EC-12, and B . breve JCM1192 were treated with or without nuclease and co-cultured with PBMCs obtained from three donors, for 24 h. The IL-12 protein concentration in the culture supernatant was measured by enzyme-linked immunosorbent assay. Data are expressed as relative percentage to non-treated LAB. The value above the bars of non-treated LAB indicates the mean value of IL-12 concentration (pg/mL) in each donor. n : non-treated, RNase A (L): RNase A treatment in low salt buffer (digests all RNA), RNase A (H): RNase A treatment in high salt buffer (digests ssRNA), Cq : chloroquine treatment of PBMCs prior to bacterial stimulation. **: P < 0.01 vs non-treated LAB, mean ± SD, n = 3 per donor for each condition.

Article Snippet: RNase III (final concentration 100 U/mL; New England Biolabs, Tokyo, Japan) that digests dsRNA was added to a high salt buffer containing 40 mg/mL of heat-killed LAB. RNase-free recombinant DNase I (Roche, Tokyo, Japan) was used for DNase treatment.

Techniques: Cell Culture, Protein Concentration, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Cells

Article Title: Fabrication Scaffold with High Dimensional Control for Spheroids with Undifferentiated iPS Cell Properties

doi: 10.3390/cells12020278

Figure Lengend Snippet: Fluorescent immunostaining results of the multidifferentiation abilities. Blue coloration shows where the cells localize, and they emit green or red (FOXA2, SOX17, PAX2, cardiac Troponin T, OTX2, OCT4, β tubulin) depending on the localization of each protein.

Article Snippet: MA, USA), mouse monoclonal anti-TRA-1-81 (1:1000; 4745, CST), mouse monoclonal anti-SSEA4 (1:1000; 4755, CST), rabbit anti-NANOG (1:500; 4903, CST), rabbit monoclonal anti-FOXA2/HNF3β (1:400; 8186S, CST), and mouse monoclonal anti-SOX17 (1:100; TA500044, Origene, Rockville, MD, USA) as early endoderm markers, rabbit polyclonal anti-Cardiac Troponin T (1:100; 15513-1-AP, Proteintech, Rosemont, IL, USA) and mouse monoclonal anti-PAX2 (1:100; MBS4380650, MyBioSourse, San Diego, CA, USA) as early ectoderm markers, and goat polyclonal anti-OTX2 (1:100; SC031B, R & D systems) and rabbit monoclonal anti-β Tubulin (1:100; 80713-1-RR, Proteintech) as early ectoderm markers.

Techniques: Immunostaining